Ketamine Promotes Alzheimer’s-Like Neurodegeneration by Activating Glycogen Synthase Kinase 3 Beta and Inhibiting Protein Phosphatase 2A

To investigate the mechanism of ketamine promoting Alzheimer’s-like neurodegeneration in mice. 24 male 8 w old Kunming mice specific pathogen-free grade were randomly divided into a blank control group (control group) and intervention (experimental according to random number table, 12 each group. The experimental given injection at dose 30 mg/kg. Mice an equal volume normal saline injection. was conducted once day for consecutive 6 mo. behavioral changes two groups compared. Western blot used detect expression levels tau (phospho Thr231), S396), Ser404), glycogen synthase kinase 3 beta, beta ser9) protein phosphatase 2A proteins brain tissue hippocampus ratio phosphorylated calculated. expressions beta-amyloid observed by immunohistochemistry. results Morris water maze showed that escape latency both gradually decreased lower than Besides, times crossing target platform proportion activity time quadrant those At same time, compared with group, Thr231) Ser404) increased. Compared significantly reduced opalescence density (p<0.05). Long-term use can lead up-regulated hippocampus, which may induce hyperphosphorylation Thr231, S396 S404 activating inhibiting 2A, causing cognitive function decline